Media in category cowpea mosaic virusthis category contains only the following file. Seed transmission of cowpea aphidborne virus in some cowpea. Assessing yield potential of cowpea genotypes grown under. Cowpea yellow mosaic virus symptoms leaf symptoms vary from green mottle to severe mosaic leaf distortion blistering and plant death control 1. This definition appears somewhat frequently and is found in the following acronym finder categories. Pdf phloem loading and unloading of cowpea mosaic virus.
Differences in the coat protein genes of cmv and blcmv. In the 50 years since it was first described, virus cpmv has become one of the most intensely studied plant viruses. The mode of gene action conferring resistance to the virus is not well understood. Tvu 2480 obtained from the international institute of. Cowpea mosaic virus article about cowpea mosaic virus by. Pdf phloem loading and unloading of cowpea mosaic virus in. We do not use these to store personal information about you. We transiently expressed, using tomato bushy stunt virus tbsv vectors and agrobacterium tumefaciens, in nearly isogenic cpacpa and cpacpa cowpea lines, sequences from rna1, the larger of two cpmv genomic rnas. This study was carried out to determine the effects of single infections and coinfections of three unrelated viruses on three cowpea cultivars one commercial cowpea cultivar white and 2 iita lines. The plants were inoculated with cowpea aphidborne mosaic virus cabmv, genus potyvirus, cowpea mottle virus cmev, genus carmovirus and southern bean mosaic virus. As a result of its ease of propagation, high yield and the stability of the viral particles, cpmv. Cowpea mosaic virus cpmv, an icosahedral 30 nm virus, offers a uniquely programmable biological nanoscaffold. It has a limited host range, is transmitted mainly by beetles and readily by sap inoculation.
General information about cowpea severe mosaic virus cpsmv0 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Some previous reports have suggested cabmv to be very closely related to blackeye cowpea mosaic virus b1cmv while other reports have concluded. These positive tests not only confirmed the presence of. Di, rong, relationships among serotypes of cowpea severe mosaic virus as determined by signature analysis 1989. Rapid detection of cowpea aphidborne mosaic virus in cowpea seeds. This thesis is submitted in partial fulfilment of the requirements of the degree of doctor of philosophy at the university of east anglia. Both rna1 and rna2 have a vpg virus genomelinked protein at the 5end, and. Engineering cowpea mosaic virus rna2 into a vector to express heterologous proteins in plants kodetham gopinath, joan wellink,1 claudine porta, kathryn m.
Natural multiple viral infections of cultivated cowpeas have been reported in nigeria. Cowpea mosaic virus nanoparticles a structure of the cowpea mosaic virus cpmv. Cowpea yellow mosaic virus chant, 1959 cowpea mosaic virus, yellow strain agrawal, 1964 an rnacontaining virus with isometric particles about 28 nm in diameter. Cowpea mosaic virus cpmv was the first plant virus to be developed as a system for the display of foreign peptides and the particles have been used extensively for chemical modification.
Phloem loading and unloading of cowpea mosaic virus in vigna unguiculata article pdf available in journal of general virology 83pt 6. The activity of defence related enzymes peroxidase and. Interference between cowpea mosaic virus and cowpea severe mosaic virus in a cowpea host immune to cowpea mosaic virus. Relationships among serotypes of cowpea severe mosaic virus as determined by signature analysis. As well as an intrinsic interest in cpmv as a plant pathogen, cpmv is of major interest in biotechnology applications such as. Cowpea aphidborne mosaic virus how is cowpea aphid. About 6% of the lines, among them line arlington, showed extreme resistance as seedlings. Aug 15, 2011 extreme resistance of arlington line cowpea vigna unguiculata to cowpea mosaic virus cpmv is under control of a dominant locus designated cpa. Molecular parameters suggest that cowpea aphidborne mosaic virus is a distinct potyvirus and bean common mosaic virus consists of at least three distinct potyviruses. Research in the past 15 to 20 years has shifted from studying the underlying genetics and structure of the virus to focusing on ways in which it can be exploited in biotechnology. Scientific advances in characterising plant viruses has led to many changes in original names and bicmv is now officially bean common mosaic virus blackeye strain, but this new name is still. The structures of a naturally empty cowpea mosaic virus. In situ vaccination with cowpea mosaic virus nanoparticles. We show that inhalation of selfassembling virus like nanoparticles from cowpea mosaic virus cpmv reduces established b16f10 lung melanoma and simultaneously generates potent systemic antitumour immunity against poorly immunogenic b16f10 in the skin.
Interactive effect of blackeye cowpea mosaic virus and cucumber. Cowpea aphidborne mosaic virus cabmv is a major virus disease in uganda that causes substantial loss of the cowpea crop especially in growth and yield. Genetic map of cowpea mosaic virus cpmv rna1 and representation of rna1. A cowpea mosaic virus nanoscaffold for multiplexed. The replication of cowpea mosaic virus, bioessays 10. Cowpea mosaic virus nanoparticles target surface vimentin. The two most important mosaicinducing viruses of cowpea in africa are blackeye cowpea mosaic virus blcmv and cowpea aphidborne mosaic virus cabmv. Differential field infection of cowpea genotypes by southern bean mosaic virus. Cowpea mosaic virus nanoparticles target surface vimentin on. As well as an intrinsic interest in cpmv as a plant pathogen, cpmv is of major interest in biotechnology applications such as nanotechnology. Selected viral families, viruses and species affected. Research in the past 15 to 20 years has shifted from studying the underlying genetics and structure of the virus to focusing on. Selected synonyms bean yellow mosaic virus, cowpea strain j. However, viral diseases, particularly cucumber mosaic virus cmv and blackeye cowpea mosaic virus blcmv, can be a limiting factor in cowpea production.
Engineering cowpea mosaic virus rna2 into a vector to. More than one thousand cowpea vigna unguiculata lines were tested for resistance to cowpea mosaic virus cpmv beier et al. The objective of the study was to determine the genetic inheritance of resistance in cowpea crosses. Pdf interactive effect of blackeye cowpea mosaic virus and. Cowpea aphidborne mosaic virus how is cowpea aphidborne mosaic virus abbreviated.
Cowpea mosaic virus cpmsv0overview eppo global database. Inheritance of resistance to blackeye cowpea mosaic virus in. We have systematically investigated how the assembly depends on three previously unaddressed. High performance liquid chromatography hplc profiles of tryptic peptides and partial amino acid sequence analysis have been employed to establish the taxonomic status of the moroccan isolate of cowpea aphidborne mosaic virus cabmv. A veinbanding strain of cowpea aphidborne mosaic virus in nigeria. The first half of this dissertation chapters deals with the in vitro selfassembly of cowpea chlorotic mottle virus ccmv from its singlestranded ss rna genome and its capsid protein cp. Viruslike particle production using cowpea mosaic virusbased vectors. Dijkstra j, bos l, bouwmeester hj, hadiastono t, lohuis h. It induces the formation of cytoplasmic cylindrical pinwheel inclusions and associated scrolls in.
In this study, three nigerian commercial cowpea cultivars olo 11, oloyin and white and two lines from the iita it86d 719 and tvu 76 were mechanically inoculated with cowpea aphidborne mosaic virus cabmv, bean southern mosaic virus sbmv and cowpea mottle virus cmev singly, as well as in all. Chemical modification of cowpea mosaic virus cpmv to create. Sep 27, 2007 natural multiple viral infections of cultivated cowpeas have been reported in nigeria. Metagenomicbased screening and molecular characterization of. Moreover, biochemical and biophysical properties of. The uvvis spectrophotometry data above shows the absorbance of the virus solutions at wavelengths ranging from 230 to 700 nm. Infection of a susceptible cowpea leaf causes a mosaic pattern in the leaf, and results in high virus yields 12 gkg. Some previous reports have suggested cabmv to be very closely related to blackeye cowpea mosaic virus b1cmv while other reports have concluded that this.
Both the wildtype cpmv and cpmvfitc virus solutions had the same virus absorbance peaks at 260 nm. Relationships among serotypes of cowpea severe mosaic. Cowpea mosaic virus cpmv is a picornalike plant virus. All structured data from the file and property namespaces is available under the. Cowpea mosaic virus, sb isolate cowpea mosaic virus cpmv is a nonenveloped plant virus of the comovirus group. Cowpea mosaic virus cpmv is a nonenveloped plant virus of the comovirus group. Cowpea mosaic virus cpmv has borne witness to most of the aforementioned biotechnological uses of rna viruses.
Lomonossoff, and ab van kammen laboratory of molecular biology, agricultural university, dreijenlaan 3, wageningen, 6703 ha, the netherlands. Cowpea mosaic virus cpmv and flock house virus fhv are excellent model viral systems for studying and engineering these viruses. Identification of blackeye cowpea mosaic virus from germplasm of yardlong bean and from soybean, and the relationships between blackeye cowpea mosaic virus and cowpea aphidborne mosaic virus. Its genome consists of 2 molecules of positivesense rna rna1 and rna2 which are separately encapsidated. The year 2009 marks the 50th anniversary of the first description of cpmv as a pathogen of cowpeas vigna unguiculata in west africa. The topics include systems based on cowpea mosaic virus for the expression of antigens and antibodies in plants, a rapid system for evaluating the bioproduction capacity of complex pharmaceutical proteins in plants, chloroplastderived vaccine antigens and biopharmaceuticals, the glycosylation of antibody therapeutics, and assessing the risk that plantderived therapeutic proteins will.
Chemical modification of cowpea mosaic virus cpmv to. Participation of the cowpea mosaic virus protease in eliciting. Passionfruitwoodiness virus, cowpea aphidborne mosaic virus, passiflora chlorosis virus, passiflora virus y. Again to our surprise, all five primers gave positive results in the two samples tested. Cowpea mosaic virus cpmv has become one of the most intensely studied plant viruses. Cowpea mosaic virus group article about cowpea mosaic. Described by anderson 1955and lima et alsome isolates taiwo et al. The image was created using chimera, the coordinates were downloaded from the viper database viperdb. Engineering and virology of the cowpea mosaic virus and flock. General information about cowpea mosaic virus cpmsv0 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. The capacity of cpmv as a simultaneous carrier for different types of. Oct 24, 2014 cowpea yellow mosaic virus symptoms leaf symptoms vary from green mottle to severe mosaic leaf distortion blistering and plant death control 1. Cowpea mosaic virus how is cowpea mosaic virus abbreviated. Engineering bsa conjugated to cpmv attenuated 99% of the virus through 10,000fold serial dilutions and conjugating rnase safely inactivated the virus.
Continuing to use this website means you agree to our use of cookies. The replication of cowpea mosaic virus the replication of cowpea mosaic virus van kammen, a eggen, h. Sep 27, 2007 in this study, three nigerian commercial cowpea cultivars olo 11, oloyin and white and two lines from the iita it86d 719 and tvu 76 were mechanically inoculated with cowpea aphidborne mosaic virus cabmv, bean southern mosaic virus sbmv and cowpea mottle virus cmev singly, as well as in all possible combinations at 10, 20 and. Presentation mode open print download current view. In the 50 years since it was first described, cowpea mosaic virus cpmv has become one of the most intensely studied plant viruses. Natural spread of the cowpea strain of southern bean mosaic virus among susceptible lines and between susceptible and resistant lines.
This study reports initial optimization of the simultaneous modification of two cpmv mutants with alexafluor 647 fluorescent dyes and either igg proteins or antibodies at specific sites on the virus scaffold. Cpsmv0 cowpea severe mosaic comovirus taxonomic tree top of page. The two most important mosaic inducing viruses of cowpea in africa are blackeye cowpea mosaic virus blcmv and cowpea aphidborne mosaic virus cabmv. Encyclopedia article about cowpea mosaic virus group by the free dictionary. Cowpea aphid borne mosaic virusmorocco and south african. Engineering and virology of the cowpea mosaic virus and. Protoplasts, recovered from the primary leaves of seedlings corresponding to these resistant lines and inoculated with cpmv, gave yields of cpmv. Viruslike particle production using cowpea mosaic virus. A cowpea mosaic virus nanoscaffold for multiplexed antibody. Genetic inheritance of resistance to cowpea aphidborne. Relationships among serotypes of cowpea severe mosaic virus. Frontiers additive interactions of unrelated viruses in. Participation of the cowpea mosaic virus protease in. This work led first to the use of virus particles to present peptides, then to the creation of a.
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